Product for plant stress reduction in agricultural crops.

Maximaizing of crop yield potential and quality.

Do you know the crop stress can severely reduce the potential yields by 65 – 87% and in extremely

harsh conditions cause so much damage it can lead to complete crop failure?

Negative effects of plant stress on plants.

Various plant stresses of plants are unavoidable throughout their growth period, which are divided into those caused by:

Abiotic stress: shortage of water (drought), too little of light, too much of light or too low temperatures, ultraviolet rays, harmful gases, plant protection products, acessive foliar fertilizers, plant nutrition deficiency, harmful heavy metals such as cadmium (Cd) lead, (Pb ), mercury (Hg), chromium (Cr), industrial waste, radioactivity, xenobiotics, saline soils, mechanical damage (strong winds, hail, etc.).

Biotic stress: diseases and insect infestations, competition with other plants, especially weeds, damage of herberivals, flowering and fruit formation period, etc.

All these environmental factors in varying degrees will limit the crop growth and reduce yield. It will also reduce the quality of the crop at harvest losing the grower money due to a reduction in yield and on top of loss of quality.

To overcome those unfavorable conditions the grower must use PROLIS ® to restore the plants physiological processes and preserve the potential yield and quality.

PROLIS® contains essential L-α-proline acid, which is essential in plants, especially under stress, plays a key role in osmoregulation, bioenergy, cell growth and reproduction and acts as an antioxidant and stimulator of enzyme formation and their precursors needed by plants maximize stress resistance. It has been found that under stress, the concentration of L-α-proline acid in the intercellular solution is significantly increased in plants, which, if the stress lasts long enough, is consumed in various physiological self-regulatory processes. In a state of stress, special chemical compounds are formed in plant cells such as non-toxic low molecular weight organic solutions, to protect against unfavorable growth conditions, and L-α-proline acid is one of the ingredients. Numerous tests have shown that its use by spraying through leaves, treatments of seeds helps effectively to protect plants from various stresses. L-α-proline acid slows down the aging process. PROLIS® improves the acidity of the intercellular solution.

PROLIS® helps to overcome plant stress caused by lack of moisture. (In drought conditions).

Water scarcity (drought) is the biggest factor limiting plant growth Worldwide, leading to a sharp drop in yields or even a total loss of yields, as well as a deterioration in crop quality. PROLIS® protects plant cells from water loss by maintaining osmotic pressure and sends a signal to the leaf stoma to shut off moisture evaporation and increases the uptake of water into the cell. PROLIS® plays a crucial role in the process of osmoregulation in cells.

PROLIS® is an antioxidant, as it helps to overcome plant stress caused by heat and too low temperatures.

Deviations from the optimal growth temperature cause significant damage to the growth and development of agricultural plants. Too hight and too low temperatures produce excessive levels of various types of reactive oxygen species (ROS), such as oxygen superoxide (O^-2), hydroxyl (OH^–), hydrogen peroxide (H₂O₂), nitrogen oxide (NO) and many others. Under normal plant growth conditions, reactive oxygen species (ROS) are within normal limits and play an important role in protecting against pathogens. Plants are more susceptible to plant diseases when in a state of stress.

The various types of reactive oxygen species (ROS) should remain within normal limits, but this is only possible under ideal plant growth conditions, which are very rare during vegetation. Under normal conditions, excess reactive oxygen species (ROS) are eliminated by protective antioxidant compounds produced by the plants themselves, but when plants are exposed to biotic and abiotic stresses, their reactive oxygen species (ROS) levels increase. Excessive oxidative processes caused by reactive oxygen species (ROS) in plants damage proteins and promote fat oxidation in cell walls, leading to electrolyte leakage, damage to nucleic acids, degradation of chlorophyll pigments, reduction of enzyme activity and damage to the entire growth chain of various physioligical functions.

PROLIS® leads to the formation of antioxidant enzymes precursors and antioxidants such as catalase, ascorbate peroxidase and superoxide dismutase and others, thus neutralize excessive amounts of reactive oxygen species (ROS) and allow plants to grow normally.

The resistance of plants to low temperatures is determined by the minimum temperature at which the plants no longer grow. For most plants, this temperature is even below + 4° C. When the plant is exposed to temperatures below zero, PROLIS® helps the plant to produce soluble sugars; as much as to 20 – 40% of them increase in plant cells, which significantly reduces the freezing temperature of the solution in the cells at lower temperatures and, as a result, increases the resistance of plants to low temperatures.

 

 

Influence of PROLIS® on photosynthesis in plants.

Any stress can damage chlorophyll and other enzymes involved in the process of photosynthesis, which means that the process of photosynthesis in plants can be disrupted and this affects the productivity of plants. PROLIS® under unfavorable to grow conditions maintains NADP⁺ (nicotinamide adenine dinucleotide phosphate) and NADPH appropriate ratio which takes an important role in energy transmission in photosynthesis. PROLIS®promotes the formation of chlorophyll B and thus makes photosynthesis more efficient. PROLIS® in wheat can increase chlorophyll content up to 40% and carotenoid content up to 50% under stress conditions. PROLIS® enhances the absorption of carbon dioxide (CO₂).

 

Influence of PROLIS® on plant growth.

PROLIS® contains an essential amino acid, which is especially important for the formation of proteins in the walls of plant cells. Those proteins are also particularly important for cell division and plant growth in general. In the event of unfavorable plant growth conditions, L-α-proline amino acid content is significantly reduced, resulting in poorer cell division, which means reduced plant growth and ungerminated seeds. Prolis® helps to optimize the amount of this essential gradient.

Influence of PROLIS® on the respiratory process in plants.

L-α-proline acid is essential in the respiratory process in plant cell mitochodria, providing energy for plants to recover more quickly from stress.

 

PROLIS® for signal transmissions.

L-α-proline acid is a signaling molecule in plants that the plant needs to protect itself from various stresses. Its higher content stimulates the synthesis of various hormones for protection against stress in plants. With PROLIS®, plants have a higher content of L-α-proline acid in the cells and recover more quickly from the effects of stress, without losing time if they produce in the plants themselves.

PROLIS® helps to heal wounds.

When plants are damaged by mechanical damage such as pests or pathogen attacs, they need to heal wounds as soon as possible. PROLIS® will help to heal the wounds.

 

PROLIS® helps to recover from stress caused by saline soils.

Stress caused by saline soils causes disturbance to nutrient metabolism and uptake by plants, especially their roots, which slows down root growth. This significantly reduces the ability of roots to absorb moisture and nutrients. Plant roots accumulate too much hydrogen peroxide (H₂O₂) in saline soils, resulting in reduced production of antioxidants. The use of PROLIS® provides osmotic protection in root cells, increasing the amount of osmolytes in them and thus optimizing the cell osmotic pressure of the root cells to absorb water and nutrients, and increasing the amount of antioxidants, thus ensuring normal plant growth. PROLIS® also promotes root and root growth, allowing plants to absorb more moisture.

 

 

 

PROLIS® improves plant nutrition.

PROLIS® improves the absorption of nutrients such as nitrogen (N), phosphorus (P), potassium (K⁺), calcium (Ca⁺), magnesium (Mg⁺) during drought, regardless of plant growth stages. PROLIS® activates more nitrogen uptake – the most important nutrient becomes vital under stress. In legumes plants significantly (up to + 50%) increases the amount of soluble protein and thus improves the quality of the crop.

 

PROLIS® eliminates plant stress caused by heavy metals.

Heavy metals that exceed normal limits, such as cadmium (Cd), lead (Pb), nickel (Ni), and chromium (Cr), are harmful to plant growth and development. In such cases, PROLIS® acts as a neutralizer of heavy metals and eliminates their harmful effects.

 

Influence of PROLIS® on nitrogenase and other enzymes and metabolites.

PROLIS® increases the formation of symbiotic bacteria on legumes plants and the activity of the enzyme nitrogenase under the drought conditions. This allows legumes to absorb more nitrogen from the air and at the same time produce more protein.

                                                                                                                                                       

PROLIS® protects against the stress caused by ultraviolet (UV) sunlight.

It is well known that plants need sunlight to ensure an active process of photosynthesis, but too much ultraviolet light can harm the plant. Ultraviolet rays promote the formation of ozone (O₃) in plants, which has high oxidative properties, and ozone severely impairs important vital processes in plants.

PROLIS®promotes the formation of antioxidants in plants that neutralize the negative effects of ozone and carotenoids that absorb or reflect ultraviolet rays and thus reduce their harmful effects.

PROLIS® helps to protect against stress caused by plant protection products.

Plant protection products, especially herbicides, can also cause stress to plants. These can be expressed as plant yellowing or chlorotic symptoms as they promote the formation of ethylene (C₂H₄), which is sigaling olecule to maturity. PROLIS® promotes the formation of antioxidants to neutralize ethylene (C₂H₄).

PROLIS® is important in disease prevention.

Plants protect themselves from disease by killing the cells around the pathogen’s hyphae, thus preventing further spread of the disease. L-α-proline acid is an especially important element in natural disease prevention. PROLIS® makes plants more resistant to disease.

PROLIS® during flowering.

During flowering, plants are suffering of biotic stress. It is found that during flowering the accumulation of L-α-proline acid in the flowers is as much as 80%, because the essential amino acid L-α-tryptophan, the precursor of which is L-α-proline acid, is needed for fruit formation. If plants are in a state of stress L-α-proline acid is consumed in other important biological processes where it is consumed to fight stress. Absence of L-α-proline acid dusing flowering, resulting in less fruit and seeds being produced. PROLIS® attracts pollinators to flowering plants, resulting in more fruit.

PROLIS® for seed treatment.

PROLIS® stimulates the formation of the enzyme alpha-amylase, which breaks down carbohydrates and converts them into the energy needed for germination and subsequent growth after germination. PROLIS® increases the germination of the crop field by promoting a more efficient use of the nutrients in the seeds. Farmers know that as soon as possible and better germinate plants can expect higher yields. PROLIS® contributes to rhizobial colonization of seedling roots.

PROLIS® impact summaries:

• Activates synthesis of antioxidant enzymes such as catalase, peroxidase, superoxide dismutase, which neutralize reactive oxygen species (ROS) under stress conditions of drought, heat and cold stress.
• Stimulates the enzyme α-amylase formation, which converts poil carboxydrates (starches) into sugars and promotes the essential amino acid L-α-ornithine formation, which is essential for seed germination.
• Important during flowering for pollination and seed formation and attraction of polinators.
• Promotes root growth during stress caused by saline soils and contributes to rhizobial colonization of seedling roots.
• Enhances cell division, which means plant growth and various other morphogenetic processes such as embryogenesis and organogenesis.
• Helps maintain osmotic pressure in cells while maintaining cell turgor and osmotic balance.
• Regulates stomata closing during drought.
• Stabilizes proteins and fats in the intercellular structure of cell walls by preventing electrolyte leakage.
• Absorbs heavy metals such as cadmium (Cd), lead (Pb), nickel (Ni) and chromium (Cr).
• Improves the formation of the enzyme nitogenase in the roots of legumes during the drought.
• Improves absorption of nutrients such as nitrogen (N), phosphorus (P), potassium (K), calcium (Ca).
• Stabilizes and improves the process of photosynthesis during stress.
• Helps plants recover from stress as they activate respiratory processes and thus release more energy.
• Helps heal wounds after mechanical damage.
• Protects plants from the harmful effects of ultraviolet (UV) sunlight.

Appivations of PROLIS®.

One package is for treatment of 5 to 10 hectares of crops!

The slurry rate is from 200 to 300 l/ha. In orchards, the standard solution rate is 500 – 1000 l/ha.

Can to be mixed with fertilizers and plant protection products if there are no restrictions on their labels.

The rate of the slurry for seed tratemnt is 10 l/t of seeds.

 

PROLIS® can be used at any time during crop vegetation to reduce plant stress.

PROLIS® can be used with plant protection and seed tratment products if there are no restrictions on the their labels.

The recommended rates for foliar spraying are from 2 to 10 g/ha. Seed treatment rate – 5 g/t.

A higher rate is recommended for higher stress, especially during droughts. The duration of exposure under stress is approximately 3 weeks, after which period PROLIS® should be used again. PROLIS®, once used, does not protect against stress throughout their growing season, so it should be used every time the plants face unfavorable growing conditions. Do not exceed the recommended rates.

Use times and rates by crop.

 

Corn	Application timing	Rate	Purpose
	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	2. With herbicides in the 6 to 8 leaf stage. (BBCH 13 – 16)	5.0 g / ha.	To eliminate herbicide induced stress and increase drought resistance.
	3. Before flowering. (BBCH 32 – 50)	5.0 g / ha.	To improve fertilization.
	4. After flowering. (BBCH 63 – 80).	5.0 g/ha.	To improve grain quality.
Legumes (soybeans, beans, peas, peas, liver beans and etc).	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy. To increase the formation of nitogenase and simbiotic bacteria on rootlets.
	2. With herbicides in the 6 to 8 leaf stage. (BBCH 10 – 30).	5.0 g/ha.	To eliminate herbicide-induced stress and increase drought resistance.
	3. Before flowering. (BBCH 30 – 60).	5.0 g/ha.	To improve seed fertilization.
	4. After flowering. (BBCH 70 – 80).	5.0 g/ha.	To improve seed quality.
Spring cereals (barley, oats, wheat, triticale)	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	3. After germination (BBCH 30-40).	2.0 g/ha.	For the reduction of stress caused by herbicides or foliar fertilizers. To increase drought resistance.
	4. At flag sheet. (BBCH 51-59).	2.0 – 5.0 g/ha.	To increase resisyance to adverse environmental factors, especially drought. To improve seed fertilization.
	5. After earing. (BBCH 71-75)	2.0 – 5.0 g / ha.	To improve grain quality.
Winter cereals (wheat, rye, barley, triticale)	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	2. In autumn after germination in combination with herbicides. (BBCH 13 – 30)	2.0 g/ha.	Sugar accumulation and improvement of overwintering.
	3. At spring after overwintering (BBCH 30-32).	2.0 g/ha.	To activate physiological processes after winter. For the reduction of stress caused by herbicides or foliar fertilizers.
	4. At flag sheet. (BBCH 51-59).	2.0 – 5.0 g/ha.	Increases resistance to adverse environmental factors, especially drought.
	5. At earing. (BBCH 71-75)	2.0 – 5.0 g/ha.	To improve grain quality.
Spring oilseed rape	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	2. After germination together with herbicides. (BBCH 10 – 30).	2.0 – 5.0 g/ha.	To reduce herbicide-induced stress and increase drought resistance.
	3. In combination with insecticides. (BBCH 30 – 40)	2.0 g/ha.	To improve drought resistance.
	4. Before flowering. (BBCH 40 – 50)	2.0 – 5.0 g/ha.	To improve seed germination and increase drought resistance.
Winter oilseed rape	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	2. In the fall after germination. (BBCH 11-19)	2.0 g ha.	To improve overwintering.
	3. At spring. (BBCH 33 – 40)	2.0 g/ha.	To improve plant regeneration drought resistance.
	4. Before flowering. (BBCH 40 – 50)	2.0 – 5.0 g/ha.	To improve seed fertilization.
Seeds fruit trees. (apples, pears, currants, raspberries, blackberries)	1. Until the leaves are fully unfolded in early spring. (BBCH 10 – 20)	5.0 to 10.0 g/ha.	To improve plant regeneration and resistance to drought or frost.
	2. Until flowering every 3 weeks in combination with fungicides or insecticides. (BBCH 20 – 70)	5.0 to 10.0 g / ha.	To improve fruit formation and increase resistance to drought and frost, to reduce fruit cracks.
	3. After harvest. (BBCH 90 – 100)	5.0 to 10.0 g / ha.	To improve overwintering.
Stone fruit trees. (Cherries, fertilizers, plums, etc.)	1. Until the leaves are fully unfolded in early spring. (BBCH 10 – 20)	5.0 to 10.0 g/ha.	To improve plant regeneration and sugar accumulation and resistance to drought or frost.
	2. Until flowering every 3 weeks in combination with fungicides or insecticides. (BBCH 20 – 70)	5.0 to 10.0 g/ha.	To improve fruit formation and increase resistance to drought and frost, to reduce fruit cracks.
	3. After harvest. (BBCH 90 – 100)	5.0 to 10.0 g / ha.	To improve overwintering.
Sugar beet.	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	2. With herbicides and fungicides. (BBCH 10 – 40).	2.0 – 5.0 g / ha.	Eliminate herbicides stress and increase drought resistance.
	3. With fungicides. (BBCH 40 – 70).	2.0 – 5.0 g / ha.	For sugar accumulation and increasing drought resistance.
Vegetables	1. Seed treatment.	5.0 g/t.	To improve seed germination and germination energy.
	2. Spraying through leaves with fungicides or insecticides.	5.0 to 10.0 g/ha.	Increasing drought and other stress resistance.
	3. Before flowering.	5.0 to 10.0 g/ha.	To improve fruit formation.
	4. After germination or transplantation	2.5 g/100 liters of water for irrigation	Increasing drought resistance, sugar accumulation, increasing fruit and seed germination.

CONSUMER RESPONSIBILITY

Recommendations for use based on trails resulst and best agricultural practices. Excessive rates can to damage the crop. The manufacturer is not responsible for conditions beyond his control, such as conditions during use, soil type, use, misture with other products. The manufacturer is not liable for damage caused by improper use of the product.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Trial data.

PROLIS® efficacy for germination of spring wheat (%).

Laboratory, 2016.

Conclusion: germination of spring wheat in laboratory tests was increased by 17%.

PROLIS® efficacy for spring wheat root length (cm).

Laboratory, 2016.

Conclusion: spring wheat root length increased by 10% 12 days after germination.

PROLIS® efficiency for spring wheat seedling height (cm).

UAB Kustodija laboratory, 2016.

Conclusion: the height of spring wheat seedlings increased by 7% after 12 days.

PROLIS® efficiency for dry weight of spring wheat seedlings.

UAB Kustodija laboratory, 2016.

Conclusion: The dry mass of spring wheat roots increased by 20% 12 days after germination.

PROLIS® efficiency for dry weight of spring wheat seedlings.

UAB Kustodija laboratory, 2016.

Conclusion: the dry weight of spring wheat seedlings increased by 20% 12 days after germination.

Fig. 6 Wheat 12 days after sowing.

Estonian Crop Research Institute, 2013. Summer oilseed rape, variety ‘Hamlet’.

Control Prolis 2 g / ha Standard preparation 2 + 2 l / ha

Yield t / ha. Yield increase t / ha

Fertilized NPK: 17-6-11 + 14S + Mg- 450 kg / ha, sown on 10/05/2013.

Conclusion: PROLIS®, applied at a rate of 2 g / ha increased the yield of spring rape by 0.93 t / ha or 32.71%.

The standard amino acid preparation applied for the first time on June 6, 2013 with the rate of 2 l / ha and for the second time on July 2, 2013 with the rate of 2 l / ha increased the yield by 0.45 t / ha or 16.35%.

Estonian Crop Research Institute, 2013. Summer oilseed rape, variety ‘Clipper’.

Control Prolis 2 g / t

Conclusion: PROLIS®,applied once 2.0 g / ha on 15/06/2013 increased the yield by 0.55 t / ha or 48.6%.

 

 

 

 

 

 

 

 

Estonian Crop Research Institute, 2013. Summer wheat, variety ‘Leni’.

1. Control 2. Prolis 5 g / t 3. Prolis 5 g / t + Lamardor 200 FF 200 ml / t.

Yield of spring wheat ‘Leni’ t / ha.

Date of sowing: 08-05-2017-05-08. Date of harvest: 01/09/2017.

Conclusion: spring wheat ‘Leni’ seeds after finishing PROLIS® Yield of 5 g / t increased by 0.44 t / ha or 7.14%.

Estonian Crop Research Institute, 2013. Spring wheat, variety ‘Uffo’.

Yield of spring wheat ‘Uffo’ t / ha.

1. Control 2. Prolis 5 g / t 3. Prolis 5 g / t + Lamardor 200 FF 200 ml / t.

Date of sowing: 08-05-2017-05-08. Date of harvest: 13/09/2017.

Conclusion: spring wheat ‘Uffo’ seeds after finishing PROLIS® Yield of 5 g / t increased by 0.31 t / ha or 6.0%.

Estonia, Rannu, 2013 field trials. Spring barley.

 

Options	Yield kg / ha
Control	6465
Prolis 2 g / ha	6800

 

Conclusion: Application of Prolis 2 g / ha BBCH 47- 49 spring barley yield increased by 335 kg / ha or 5.1%.

Estonian Crop Research Institute, 2014. Spring wheat.

Options	Yield t / ha, (14%)	Saiko weight g / l
1. Control.	4,525	739.9
2. ProlisTM 5 g / t.	4,769	745.3
R05	0.183	4.16

Conclusion: After seedingPROLIS® The yield of 5 g / t increased 224 kg / ha or 5.4%.

Estonian Crop Research Institute, 2016. Winter wheat ‘Fredis’.

Options	Harvest kg / ha	1000 seeds weight	Harvest Appendix kg
1. Control	4742	42.0
2. Prole ™ 5 g / t stained.	5089	42.4	+347
3. Prolis ™ 5 g / t stained + Prolis ™ 2 g / ha sprayed in autumn + Prolis ™ 2 g / ha is sprayed in spring.	5230	42.8	+488

 

Yield t / ha.

Test scheme: 1. Control. 2. Prole stained 5 g / t 3. Prole stained 5 g / t + sprayed in autumn 2 g / ha + spring 2 g / ha.

Conclusion: after finishing the seeds PROLIS® The yield of 5 g / t increased347 kg / ha or 7.32%. After finishing the seedsPROLIS® The application of 5 g / t in autumn and 2 g / ha in spring and the resumption of vegetation in spring increased the yield of winter wheat ‘Fredis’ by 488 kg / ha or 10.2%. Prolis ™ increased the weight of 1000 seeds.

 

 

Estonian Crop Research Institute, 2016. Winter wheat ‘Kallas’.

Options	Yield kg / ha	Weight of 1000 grains in grams	Harvest supplement kg
Control	5836	45.5
Stained Baritone Ultra 0.5 l / t + Prolis 5 g / t	6516	45.6	+680

Conclusion: After sowing the seeds of winter wheat variety ‘Kallas’, the yield of Bariton Ultra 0.5 l / t and Prolis 5 g / ha increased by 680 kg / ha or 11.65%.

Options	Delius kg / ha	1000 Grain weight in grams.	Harvest supplement kg
1. Control.	4742	42.0
2. Prolis 5 g / t stained.	5089	42.4	+347
3. Prolis ™ 5 g / t stained + Prolis ™ 2 g / ha sprayed in autumn + Prolis ™ 2 g / ha sprayed in spring.	5230	42.8	+488

Estonian Crop Research Institute, 2017. Winter wheat ‘Kallas’.

Options	Sprayed 11/05/2017 (BBCH 13-15)	Sprayed 2017-04-28 (BBCH 25)	Harvest t / ha.	% Of small grains	Saiko weight g / l.	1000 Grain weight g
1. Control.			4,305	36.1	786	42.8
2. ProlisTM 5 g / t Stained.	ProlisTM 2 g / ha	ProlisTM 2 g / ha	5,830	23.7	794	43.6

Conclusion: after seedingPROLIS® 5 g / t and the application of 2 g / ha in autumn and 2 g / ha in spring after the resumption of vegetation in spring increased the yield of winter wheat ‘Kallas’ by 1.55 t / ha or 35.5%. When used in combination with fungicides, the yield increased by 1.73 t / ha or 40.1%.

Estonian Crop Research Institute, 2018. Winter wheat xxx ‘.

Options	Use time			Yield kg / ha	Yield kg / ha	Yield increase%	Saiko weight g / l	1000 grain mass g
	2018-05-18	2018-05-21	2018-05-28
	BBCH 20	BBCH 33	BBCH 43
Control				4039			829.2	46.3
Baritone Ultra 0.5 l / t + Prolis 5 g / t	Prolis 2 g / ha	Prolis 2 g / ha	Prolis 2 g / ha	5109	1070	26	844.9	47.5

Conclusion:2018 was very dry. The largest delays were obtained by applying the fungicide twice

Prolis used after finishing and spraying 3 times increased the yield of winter wheat by 26%.

Mironovsky Wheat Institute, Ukraine, 2016.

Options	Harvest t / ha.	Yield increase t / ha.	Sedimentation index.	Protein%.	Gluten%
Variety ‘Simokoda Mironovska’.
1. Control.	4.69	–	55	14.2	25.1
2. ProlisTM 2 g / ha (End of bushing).	4.87	0.18	65	14.3	25.1
3. ProlisTM 2 g / ha (flag leaf).	5.14	0.45	65	14.4	27.4
4. ProlisTM 2 g / ha (flowering).	4.98	0.29	66	14.9	29.4
Breed ‘Ponianka’.
1. Control.	4.47	–	59	13.8	26.3
2. ProlisTM 2 g / ha (end of bushing).	4.63	0.16	60	13.8	27.0
3. ProlisTM 2 g / ha (flag leaf).	4.88	0.41	66	14.1	28.3
4. ProlisTM 2 g / ha (flowering).	4.70	0.23	66	14.7	30.8
LSD 0.05%		0.18

Mironovsky Wheat Institute, 2016.

A sequence number.	Options	Harvest t / ha.	1000 Grain weight grams.	Protein%.	Glutenim%.
	Breed ‘Oberig Mironovskij’
1	Control	3.30	31.2	11.3	26.5
2	Stained Prolis 5 g / t.	3.50	32.4	11.7	26.9
3	Stained Maxim Star 035 FS 1.5 l / t.	3.55	32.6	11.3	26.8
4	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t.	3.63	33.1	11.8	27.3
5	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 11-12.	3.80	34.0	12.0	27.9
6	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 21-25.	3.90	34.5	12.3	28.6
7	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 51-59.	3.92	35.2	12,	29.5
8	Stained Maxim Star 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 71-72.	3.85	35.0	12.7	29.4
9	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis sprayed at 2 g / ha at BBCH 21-25, 51-59.	4.02	36.6	12.2	29.3
10	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 11-12, 21-25, 51-59, 71-92.	4.10	36.7	12.5	29.9
Berieginia Mironovska
1	Control	4.03	33.6	11.5	26.0
2	Stained Prolis 5 g / t.	4.15	33.8	11.6	26.5
3	Stained Maxim Star 035 FS 1.5 l / t.	4.16	33.9	11.5	26.4
4	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t.	4.33	34.1	11.6	26.3
5	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 11-12.	4.37	35.2	11.7	26.7
6	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 21-25.	4.62	36.7	11.8	26.9
7	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 51-59.	4.64	36.8	11.7	27.1
8	Stained Maxim Star 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 71-72.	4.41	36.4	12.2	28.1
9	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis sprayed at 2 g / ha at BBCH 21-25, 51-59.	4.65	37.1	12.0	28.3
10	Stained Maxim Star 035 FS 1.5 l / t + Stained Prolis 5 g / t + Prolis is sprayed at 2 g / ha BBCH 11-12, 21-25, 51-59, 71-92.	4.78	37.5	12.4	28.7
LSD 05		0.344	1.3

Conclusion: PROLISTM increased yields by 0.750 – 0.80 t / ha or 18 – 24% <protein increased by 0.9-1.2% or 7.8 – 12.4% – and gluten content increased by 2.4 – 3.4% or 10, 4 -12.83%.

Field demonstration trials in Ukraine in 2018.

Elements of crop structure.	Location Rivne		Location Chmielnitskij		Location Bravory
	Winter wheat variety ‘Skagen’		Winter wheat		Winter wheat
	Control	PROLIS ™	Control	PROLIS ™	Control	PROLIS ™
Productive stems, pcs / m2.	377	380	360	365	358	361
Scrub coefficient:	1.3	1.3	1.4	1.4	1.2	1.5
Average number of bells.	19.1	19.3	18.8	19.4	19.5	20.2
Number of bells with grain	15.1	16.3	15.8	16.6	16	16.5
Average amount of grain in the penis pcs.	36.5	38.4	31.4	34.7	34	35.3
Grain weight in the penis in grams.	1.7	1.9	1.6	1.8	1.5	1.6
Plant weight g / m2.	850.4	980.7	851.9	1816.2	514.8	675.6
1,000 grain weight g.	47.5	48.8	50	52	43.5	46.1
Biological yield t / ha.	6.5	7.1	5.7	6.5	5.3	5.8

Elements of crop structure.	Location Bravory		Location Letkivka		Location Khmielnitsky
	Winter Wheat		Winter Wheat		Spring wheat
	Check	PROLIS	Check	PROLIS	Check	PROLIS
Productive stems, pcs / m2.	358	361	364	370	228	235
Tillering coefficient:	1.2	1.5	1.6	1.5	2.1	2.1
Ear length cm.	8.5	8.7	6.3	7.9	5.3	5.6
Average number of bells.	19.5	20.2	16	18.8	12.6	12.6
Number of bells with grain	16	16.5	14	17	12.2	12.4
The average amount of grain in the penis	34	35.3	39.2	41.5	30.3	34
Grain weight in the penis in grams.	1.5	1.6	1.4	2.1	2.5	1.9
Plant weight g / m2.	514.8	675.6	426.8	746.9	777	713.6
1,000 grain weight g.	43.5	46.1	42.8	44.4	78.4	57.3
Biological yield t / ha.	5.3	5.8	6.1	6.8	5.4	4.6

Buchach Chlebokombinat AO Laboratory, Ukraine, 2018.

	Options	Average seedling height cm	Number of leaflets pcs.	Average root length cm	Bushing
Winter wheat variety Cube	PROLIS	15.25	3	9.75	1
	Control	9.5	1	7.5	1
Winter barley variety ‘Luran’	PROLIS	14.5	7	10.0	3
	Control	12.75	3	9.25	1

Laboratory tests showed that the average height of winter wheat seedlings of the ‘Kubus’ variety, the seeds of which were treated with PROLIS, was 60.5% higher than that of the untreated ones, and the average root length was 30% longer.

The average seedling height of ‘Luran’ winter barley seed treated with PROLIS was 13.72% higher than that of untreated and the average root length was 8.1% higher. than untreated seeds.

 

 

 

 

 

 

 

 

 

Laboratory germination rate tests of winter wheat seeds. Ukraine 2018.

On 25.05.2018, at the laboratory of LLC „Buchachahrohlibprom“ a study on seed germination of winter wheat using Prolis.

Impact of Prolis to germination rate.

                  

Treatments	Germination rate in % after 4 days	Germination rate in % after days	Germination rate in % after  12 days	The increase in the % after 12 days
CONTROL	94.33	95.33	95.33	–
Prolis® 5 g/ton	94.66	96.66	96,00	+0.97

 

Conclusion: Treatment of winter wheat with and Prolis, 5 g /ton increased germination rate by 0.97 %, compared with control.

 

Aleksandras Stulginskis University, 2014. Winter rape.

1. 2. Prole 2.0 g / ha in autumn. 3. Prolis 2.0 g / ha in autumn + Prolis 2.0 g / ha in spring. 4. Prolis 4.0 g / ha in autumn + Prolis 4.0 g / ha in spring.

 

Alexander Stulginsky University, 2015. Winter oilseed rape.

Test scheme:

2. Prolis applied in autum 2 g / ha.
3. Prolis applied in autum 4 g / ha.
4. Prolis in autumn 2 g / ha and Prolis in spring 2 g / ha.
5. Prolis in autumn 4 g / ha and Prolis in spring 4 g / ha.
6. Prolis in autumn 4 g / ha (5-6 leaf stage) and Prolis in autumn 4 g / ha (7-8 leaf stage).

Conclusion: Winter oilseed rape overwintered best using Prolis 4 gr / ha in spring and 4 g / ha in autumn.

Conclusion: The highest winter rapeseed yield was after one application – 2 g / ha in autumn.

Yield t / ha

1 2 3 4 5 6

Productivity of one plant in grams

Conclusion: The productivity of winter oilseed rape per plant was the highest after a single application – 4 g / ha in autumn.

Weight of 1000 seeds in grams

Conclusion: The weight of 1000 seeds of winter rape was the highest after one application – 4 g / ha in autumn.

Aleksandras Stulginskis University, 2014. Sugar beet.

Yield t / ha

1.      Control

           

 

 

 

 

 

 

Aleksandras Stulginskis University, 2014. Sugar beet.

	Yield t / ha	Sugar content%	% Increase in sugar content	Yield increase t / ha	Additional sugar curse t / ha
Control	6,510	16.90
ProlisTM – 2 g / ha	7,140	17.20	0.30	6.3	1.08
ProlisTM – 2 + 2 g / ha	7,210	17.30	0.40	7.0	1.21
ProlisTM – 2 + 2 + 2 g / ha	7,430	17.50	0.60	9.2	1.61

Conclusion: After application of Prolis 2 once 2 g / ha additional 1.08 t / ha of pure sugar was obtained, twice application of 2 g / ha additional 1.21 t / ha, and three applications of 2 g / ha additional 1.61 pure sugar.

Brazil, 2018, Maranhao, San Raimundo area. RIZA test field. Soybean variety ‘M808’.

Options	Germination	Plant height cm	Yield kg / ha
1.      Control	76.92%	65	3780
2.      Stained Prolis 5 g / ha	87.12%
3.      Sprayed with Prolis 5 g / ha + 5 g / ha		68	3900

Conclusion: Soybean field germination increased by 13.2% and yield by 120 kg / ha or 3.1%.

Upinhal University of Agriculture. Department of Agronomy. Brazil. 2018.

Precision tests in vegetation pots. The first spray is germinated and the second 15 days after the first spray.

Test scheme:

1. Control.
2. Stained 2 g / t
3. Stained 4 g / t.
4. Spray 2 g / ha.
5. Spray 4 g / ha.
6. Stained 2 g / t + Spray 2 g / ha.
7. Stained 2 g / t + Spray 4 g / ha.
8. Stained 4 g / t + Spray 2 g / ha.
9. Stained 4 g / t + Spray 4 g / ha.

Results:

Plant weight in grams

Conclusions:The use of prole in staining at 2 g / t and 4 g / t increased the weight of plants from 33% to 50%. A lower rate was more effective. After one application, 2 g / ha of plant weight was increased by 206%.

Pod weight in grams

Conclusions: Lower Prolis rates for staining were more effective. After sowing the seeds at a rate of 2 g / ha increased the weight of the pods by 74%. Higher rates were more effective when spraying through the leaves. The weight of the pods increased by 80% after 4 g / ha.

Programmed cell death proteosis

Intercellular fluid

Absorbs more CO2

6